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DD–RT–PCR identifies 7-dehydrocholesterol reductase as a key marker of early Leydig cell steroidogenesis

Anbalagan, M and Yashwanth, R and Rao, Jagannadha A (2004) DD–RT–PCR identifies 7-dehydrocholesterol reductase as a key marker of early Leydig cell steroidogenesis. In: Molecular and Cellular Endocrinology, 219 (1-2). pp. 37-45.

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Postnatal Leydig cell development in rat involves an initial phase of proliferation of progenitor Leydig cells (PLCs) and subsequent differentiation of these cells into immature Leydig cells (ILCs) and adult Leydig cells (ALCs). With an objective to identify the molecular changes associated with Leydig cell differentiation, the mRNA population in PLCs and ILCs were analyzed by the technique of differential display reverse transcription polymerase chain reaction (DD–RT–PCR). Results revealed differential expression of several transcripts in PLCs and ILCs. Of the several differentially expressed transcripts, the expression of transcripts corresponding to collagen IV $\alpha$6 (Col IV $\alpha$6) and ribosomal protein L 41 (RpL41) decreased during the differentiation of PLC to ILC. Also there was an increase in the expression of transcripts encoding enzymes such as microsomal glutathione-S-transferase (mGST 1) and 7-dehydrocholesterol reductase (7-DHCR) during this process. While Col IV $\alpha$6 and RpL41 are known to be involved in cellular proliferation, mGST 1 and 7-DHCR are essential for normal Leydig cell steroidogenesis. A detailed study on 7-DHCR expression in Leydig cells revealed that this enzyme plays a crucial role in steroidogenesis. Interestingly expression of this enzyme is not under acute regulation by Luteinizing hormone (LH).

Item Type: Journal Article
Publication: Molecular and Cellular Endocrinology
Publisher: Elsevier
Additional Information: The copyright belongs to Elsevier.
Keywords: Leydig cell;DD–RT–PCR;Col IV α6;RpL41;mGST 1;7-DHCR
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 08 May 2006
Last Modified: 19 Sep 2010 04:26
URI: http://eprints.iisc.ac.in/id/eprint/6603

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