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Formation of a DNA Layer on Langmuir-Blodgett Films and Its Enzymatic Digestion

Bhaumik, Anusarka and Ramakanth, Madhugiri and Brar, Loveleen Kaur and Raychaudhuri, Arup K and Rondelez, Francis and Chatterji, Dipankar (2004) Formation of a DNA Layer on Langmuir-Blodgett Films and Its Enzymatic Digestion. In: Langmuir, 20 (14). pp. 5891-5896.

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Here,wereport a systemwehave developed where long double-strandedDNAs(dsDNAs) are immobilized on a monolayer of Zn-arachidate.Wehave applied the Langmuir-Blodgett technique to form the monolayer ofZn-arachidate where Zn(II) is bound to arachidic acid through charge neutralization. Because tetrahedral Zn(II) participates in DNA recognition through coordination, we have been able to layer DNA over the Zn-arachidate monolayer. The DNA layer shows a typical compression and expansion cycle in a concentration-dependent fashion. Interestingly, theDNAmonolayer is available for enzymatic degradation by DNaseI. The detection of DNA and its accessibility towards biological reaction is demonstrated by imaging through fluorescence microscopy. The conformation of the DNA, immobilized on the monolayer, was studied with the help of atomic force microscopy (AFM). We observed that the dsDNAs were aligned in a stretched manner on the surface. To investigate further, we also demonstrate here that the small single-stranded DNA (ssDNA) immobilized on the air-water interface can act as a target molecule for the complementary ssDNA present in the subphase. The study of DNA hybridization done with the help of fluorescence spectroscopy clearly supports the AFM characterization.

Item Type: Journal Article
Publication: Langmuir
Publisher: American Chemical Society
Additional Information: The copyright belongs to American Chemical Society.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Physical & Mathematical Sciences > Physics
Date Deposited: 10 May 2006
Last Modified: 19 Sep 2010 04:26
URI: http://eprints.iisc.ac.in/id/eprint/6599

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