Dual cross-linked honey coupled 3D antimicrobial alginate hydrogels for cutaneous wound healing

Mukhopadhyay, A and Rajput, M and Barui, A and Chatterjee, SS and Pal, NK and Chatterjee, J and Mukherjee, R (2020) Dual cross-linked honey coupled 3D antimicrobial alginate hydrogels for cutaneous wound healing. In: Materials Science and Engineering C, 116 .

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Abstract

We report potentiation of healing efficacy of alginate by value addition at its structural level. Dual crosslinked (ionically and covalently) sodium alginate hydrogel coupled with honey (HSAG) brings about an intermediate stiffness in the fabric, confers consistent swelling property and limits erratic degradation of the polymer which ultimately provides conducive milieu to cellular growth and proliferation. In this work honey concentrations in HSAGs are varied from 2 to 10. FTIR, XRD and nanoindentation studies on the HSAGs exhibited physicochemical integrity. In vitro degradation study provided the crucial finding on 4 HSAG having controlled degradation rate up to 12 days with a weight loss of 87.36 ± 1.14. This particular substrate also has an ordered crystalline surface morphology with decent cellular viability (HaCaT and 3T3) and antimicrobial potential against Methicillin Resistant Staphylococcus aureus (MRSA) and Escherichia coli. The in vivo wound contraction kinetics on murine models (4 HSAG treated wound contraction: 94.56 ± 0.1) has been monitored by both invasive (histopathology) and noninvasive (Swept Source Optical Coherence Tomography) imaging and upon corroborating them it evidenced that 4 HSAG treated wound closure achieved epithelial thickness resembling to that of unwounded skin. Thus, the work highlights structurally modified alginate hydrogel embedded with honey as a potential antimicrobial healing agent. © 2020 Elsevier B.V.

Item Type:	Journal Article
Publication:	Materials Science and Engineering C
Publisher:	Elsevier Ltd
Additional Information:	Copy right for this article belongs to Elsevier Ltd
Keywords:	Biodegradation; Degradation; Escherichia coli; Food products; Hydrogels; Morphology; Optical tomography; Sodium alginate; Staphylococcus aureus; Surface morphology; Tissue regeneration, Alginate hydrogels; Cellular viability; Controlled degradation; Crystalline surfaces; Epithelial thickness; Methicillin-resistant staphylococcus aureus; Swelling properties; Swept source optical coherence tomographies, Antimicrobial agents
Department/Centre:	Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	14 Oct 2020 11:29
Last Modified:	14 Oct 2020 11:29
URI:	http://eprints.iisc.ac.in/id/eprint/65912

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