Single-Cell Analysis Reveals Partial Reactivation of X Chromosome instead of Chromosome-wide Dampening in Naive Human Pluripotent Stem Cells

Mandal, S and Chandel, D and Kaur, H and Majumdar, S and Arava, M and Gayen, S (2020) Single-Cell Analysis Reveals Partial Reactivation of X Chromosome instead of Chromosome-wide Dampening in Naive Human Pluripotent Stem Cells. In: Stem Cell Reports, 14 (5). pp. 745-754.

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Abstract

Recently, a unique form of X chromosome dosage compensation has been demonstrated in human preimplantation embryos, which happens through the dampening of X-linked gene expression from both X chromosomes. Subsequently, X chromosome dampening has also been demonstrated in female human pluripotent stem cells (hPSCs) during the transition from primed to naive state. However, the existence of dampened X chromosomes in both embryos and hPSCs remains controversial. Specifically, in preimplantation embryos it has been shown that there is inactivation of X chromosome instead of dampening. Here, we performed allelic analysis of X-linked genes at the single-cell level in hPSCs and found that there is partial reactivation of the inactive X chromosome instead of chromosome-wide dampening upon conversion from primed to naive state. In addition, our analysis suggests that the reduced X-linked gene expression in naive hPSCs might be the consequence of erasure of active X chromosome upregulation. © 2020 The Authors In this article, Gayen and colleagues show that conversion of primed human pluripotent stem cells (hPSCs) to naive state is associated with partial reactivation of inactive X, not X dampening, and erasure of active X upregulation can lead to the reduction in X-linked gene expression in naive hPSCs. © 2020 The Authors

Item Type:	Journal Article
Publication:	Stem Cell Reports
Publisher:	Cell Press
Additional Information:	Copy right for this article belongs to Cell Press
Keywords:	article; cell level; embryo; human; human cell; human embryo; pluripotent stem cell; preimplantation embryo; single cell analysis; single cell RNA seq; upregulation; X chromosome inactivation
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	01 Oct 2020 11:26
Last Modified:	01 Oct 2020 11:26
URI:	http://eprints.iisc.ac.in/id/eprint/65599

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