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Elucidation of the RNA-granule inducing sodium azide stress response through transcriptome analysis

Garg, M and Poornima, G and Rajyaguru, PI (2020) Elucidation of the RNA-granule inducing sodium azide stress response through transcriptome analysis. In: Genomics, 112 (5). pp. 2978-2989.

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Official URL: https://dx.doi.org/10.1016/j.ygeno.2020.05.001


Sodium azide is a commonly used cytochrome oxidase inhibitor that leads to translation repression and RNA granule assembly. The global changes in mRNA abundance in response to this stressor are unknown. RGG-motif proteins Scd6 and Sbp1 are translation-repressors and decapping-activators that localize to and affect the assembly of RNA granules in response to sodium azide stress. Transcriptome-wide effects of these proteins remain unknown. To address this, we have sequenced transcriptome of the: a) wild type strain under unstressed and sodium azide stress, b) Δscd6 and Δsbp1 strains under unstressed and sodium azide stress. Transcriptome analysis identified altered abundance of many transcripts belonging to stress-responsive pathways which were further validated by qRT-PCR results. Abundance of several transcripts was altered in Δscd6/Δsbp1 under normal conditions and upon stress. Overall, this study provides critical insights into transcriptome changes in response to sodium azide stress and the role of RGG-motif proteins in these changes. © 2020 The Authors

Item Type: Journal Article
Publication: Genomics
Publisher: Academic Press Inc.
Additional Information: Copy right for this article belongs to Academic Press Inc.
Keywords: repressor protein; RNA; sbp1 protein; scd6 protein; sodium azide; transcriptome; unclassified drug, Article; cell stress; controlled study; fungal cell culture; microscopy; nonhuman; oxidative stress; priority journal; protein expression; protein motif; real time reverse transcription polymerase chain reaction; sequence analysis; transcriptomics; wild type
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 12 Nov 2020 06:38
Last Modified: 12 Nov 2020 06:38
URI: http://eprints.iisc.ac.in/id/eprint/65573

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