Systemic and oral immunogenicity of hemagglutinin protein of rinderpest virus expressed by transgenic peanut plants in a mouse model

Khandelwal, Abha and Renukaradhya, GJ and Rajasekhar, M and Sita, Lakshmi G and Shaila, MS (2004) Systemic and oral immunogenicity of hemagglutinin protein of rinderpest virus expressed by transgenic peanut plants in a mouse model. In: Virology, 323 (2). pp. 284-291.

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Abstract

Rinderpest causes a devastating disease, often fatal, in wild and domestic ruminants. It has been eradicated successfully using a live, attenuated vaccine from most part of the world leaving a few foci of disease in parts of Africa, the Middle East, and South Asia. We have developed transgenic peanut (Arachis hypogaea L.) plants expressing hemagglutinin (H) protein of rinderpest virus (RPV), which is antigenically authentic. In this work, we have evaluated the immunogenicity of peanut-expressed H protein using mouse model, administered parenterally as well as orally. Intraperitoneal immunization of mice with the transgenic peanut extract elicited antibody response specific to H. These antibodies neutralized virus infectivity in vitro. Oral immunization of mice with transgenic peanut induced H-specific serum IgG and IgA antibodies. The systemic and oral immunogenicity of plant-derived H in absence of any adjuvant indicates the potential of edible vaccine for rinderpest.

Item Type:	Journal Article
Publication:	Virology
Publisher:	Elsevier
Additional Information:	The copyright belongs to Elsevier.
Keywords:	Rinderpest virus;Hemagglutinin protein;Transgenic Arachis hypogaea;Oral immunogenicity
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	26 Apr 2006
Last Modified:	19 Sep 2010 04:26
URI:	http://eprints.iisc.ac.in/id/eprint/6493

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