Pathogenic Escherichia coli (E. coli) detection through tuned nanoparticles enhancement study

Satpathy, G and Chandra, GK and Manikandan, E and Mahapatra, DR and Umapathy, S (2020) Pathogenic Escherichia coli (E. coli) detection through tuned nanoparticles enhancement study. In: Biotechnology Letters, 42 (5). pp. 853-863.

PDF bio_let_42-05_853-863_2020.pdf - Published Version Restricted to Registered users only Download (901kB) | Request a copy

Abstract

Objective: This study aims to detect pathogenic Escherichia coli (E. coli) bacteria using non-destructive fluorescence microscopy and micro-Raman spectroscopy. Results: Raman vibrational spectroscopy provides additional information regarding biochemical changes at the cellular level. We have used two nanomaterials zinc oxide nanoparticles (ZnO-NPs) and gold nanoparticles (Au-NPs) to detect pathogenic E. coli. The scanning electron microscope (SEM) with energy dispersive X-ray (EDAX) spectroscopy exhibit surface morphology and the elemental composition of the synthesized NPs. The metal NPs are useful contrast agents due to the surface plasmon resonance (SPR) to detect the signal intensity and hence the bacterial cells. The changes due to the interaction between cells and NPs are further correlated to the change in the surface charge and stiffness of the cell surface with the help of the fluorescence microscopic assay. Conclusions: We conclude that when two E. coli strains (MTCC723 and MTCC443) and NPs are respectively mixed and kept overnight, the growth of bacteria are inhibited by ZnO-NPs due to changes in cell membrane permeability and intracellular metabolic system under fluorescence microscopy. However, SPR possessed Au-NPs result in enhanced fluorescence of both pathogens. In addition, with the help of Raman microscopy and element analysis, significant changes are observed when Au-NPs are added with the two strains as compared to ZnO-NPs due to protein, lipid and DNA/RNA induced conformational changes.

Item Type:	Journal Article
Publication:	Biotechnology Letters
Publisher:	Springer
Additional Information:	The copyright of this article belongs to Springer
Keywords:	Bacteria; Cell membranes; Cytology; Escherichia coli; Fluorescence; Fluorescence microscopy; Fluorescence spectroscopy; Gold nanoparticles; II-VI semiconductors; Metal nanoparticles; Morphology; Pathogens; Scanning electron microscopy; Surface morphology; Surface plasmon resonance; Zinc oxide, Cell membrane permeability; E. coli; Micro Raman Spectroscopy; Pathogenic Escherichia coli; Raman; Raman vibrational spectroscopy; The scanning electron microscopes (SEM); Zinc oxide nanoparticles, ZnO nanoparticles
Department/Centre:	Division of Chemical Sciences > Inorganic & Physical Chemistry Division of Mechanical Sciences > Aerospace Engineering(Formerly Aeronautical Engineering)
Date Deposited:	16 Jun 2020 10:50
Last Modified:	16 Jun 2020 10:50
URI:	http://eprints.iisc.ac.in/id/eprint/64724

Actions (login required)

View Item