A reversible, benzothiazole-based �Turn-on� fluorescence sensor for selective detection of Zn2+ ions in vitro

Musib, D and Raza, MK and Devi, SS and Roy, M (2020) A reversible, benzothiazole-based �Turn-on� fluorescence sensor for selective detection of Zn2+ ions in vitro. In: Journal of Chemical Sciences, 132 (1).

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Abstract

Abstract: Temperature-driven, highly sensitive and selective �TURN-ON� fluorometric detection of Zn2+ by benzothiazole-based probes (L1 and L2) was reported in physiological pH in the present work. Iron(II) acted as the reversible switch or trigger for the reversible detection of Zn2+ ions by the probes resulting in �TURN-OFF� fluorescence at room temperature. However, selective detection of Zn2+ in the presence of Fe2+ was irreversible at 0�5 °C. Such temperature dependence on reversible fluorometric detection of Zn2+ in the presence of Fe2+ was explained from the thermodynamic perspective as well as DFT calculations in which the absolute enthalpy (H) and Gibbs free energy (G) of the resultant complexes and the fluorophores (L1 and L2) at different temperatures were determined. Enhanced fluorescence of the Zn2+ bound probes was due to the inhibition of excited-state intramolecular proton transfer (ESIPT). Effect of solvents, pH, and temperature on the fluorometric detection of Zn2+ was also probed in the present work. The results were translated into the visual detection of Zn2+ on paper-based fluorescence probe and later we demonstrated the sensing of mobile Zn2+ ions by the probes in living HeLa cells as the proof of concept of our present investigations.

Item Type:	Journal Article
Publication:	Journal of Chemical Sciences
Publisher:	Springer
Additional Information:	The copyright of this article belongs to Springer
Keywords:	Excited states; Fluorescence; Free energy; Gibbs free energy; Ions; Lanthanum compounds; pH effects; Probes; Temperature distribution, Cellular imaging; DFT and TD-DFT; Reversible switches; Selective detection; Site screening; Temperature dependence, Iron compounds
Department/Centre:	Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	06 Apr 2021 09:44
Last Modified:	06 Apr 2021 09:44
URI:	http://eprints.iisc.ac.in/id/eprint/64681

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