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Saccharomyces cerevisiae Hop1 Zinc Finger Motif Is the Minimal Region Required for Its Function in Vitro

Anuradha, S and Muniyappa, K (2004) Saccharomyces cerevisiae Hop1 Zinc Finger Motif Is the Minimal Region Required for Its Function in Vitro. In: Journal of Biological Chemistry, 279 (28). pp. 28961-28969.

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Saccharomyces cerevisiae meiosis-specific HOP1, which encodes a core component of synaptonemal complex, plays a key role in proper pairing of homologous chromosomes and processing of meiotic DNA double strand breaks. Isolation and analysis of hop1 mutants indicated that these functions require $Cys^{371}$ of Hop1 embedded in a region (residues 343–378) sharing homology to a zinc finger motif (ZnF). However, the precise biochemical function of Hop1, or its putative ZnF, in these processes is poorly understood. Our previous studies revealed that Hop1 is a DNA-binding protein, showed substantially higher binding affinity for G4 DNA, and enhances its formation. We report herein that ZnF appears to be sufficient for both zinc as well as DNA-binding activities. Molecular modeling studies suggested that Hop1 ZnF differs from the previously characterized natural ZnFs. The zinc-binding assay showed that the affinity for zinc is weaker for C371S ZnF mutant compared with the wild type (WT) ZnF. Analysis of CD spectra indicated that zinc and DNA induce substantial conformational changes in WT ZnF, but not in C371S ZnF mutant. The results from a number of different experimental approaches suggested that the DNA-binding properties of ZnF are similar to those of full-length Hop1 and that interaction with DNA rich in G residues is particularly robust. Significantly, WT ZnF by itself, but not C371S mutant, was able to bind duplex DNA and promote interstitial pairing of DNA double helices via the formation of guanine quartets. Together, these results implicate a direct role for Hop1 in pairing of homologous chromosomes during meiosis.

Item Type: Journal Article
Publication: Journal of Biological Chemistry
Publisher: The American Society for Biochemistry and Molecular Biology
Additional Information: The copyright belongs to The American Society for Biochemistry and Molecular Biology.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 25 Apr 2006
Last Modified: 19 Sep 2010 04:25
URI: http://eprints.iisc.ac.in/id/eprint/6456

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