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Control of locomotory behavior of caenorhabditis elegans by the immunoglobulin superfamily protein RIG-3

Bhardwaj, A and Pandey, P and Babu, K (2020) Control of locomotory behavior of caenorhabditis elegans by the immunoglobulin superfamily protein RIG-3. In: Genetics, 214 (1). pp. 135-145.

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Official URL: https://dx.doi.org/10.1534/genetics.119.302872


Cell surface immunoglobulin superfamily (IgSF) proteins play important roles in the development and function of the nervous system . Here we define the role of a Caenorhabditis elegans IgSF protein, RIG-3, in the function of the AVA command interneuron. This study reveals that RIG-3 regulates the abundance of the glutamate receptor subunit, GLR-1, in the AVA command interneuron and also regulates reversal behavior in C. elegans. The mutant strain lacking rig-3 (rig-3 (ok2156)) shows increased reversal frequency during local search behaviors. Genetic and behavioral experiments suggest that RIG-3 functions through GLR-1 to regulate reversal behavior. We also show that the increased reversal frequency seen in rig-3 mutants is dependent on the increase in GLR-1 abundance at synaptic inputs to AVA, suggesting that RIG-3 alters the synaptic strength of incoming synapses through GLR-1. Consistent with the imaging experiments, altered synaptic strength was also reflected in increased calcium transients in rig-3 mutants when compared to wild-type control animals. Our results further suggest that animals lacking rig-3 show increased AVA activity, allowing the release of FLP-18 neuropeptide from AVA, which is an activity-dependent signaling molecule. Finally, we show that FLP-18 functions through the neuropeptide receptor, NPR-5, to modulate reversal behavior in C. elegans.

Item Type: Journal Article
Publication: Genetics
Publisher: Genetics Society of America
Additional Information: Copyright of this article belongs to Genetics Society of America
Keywords: AVA; Caenorhabditis elegans; GLR-1; Neuropeptide; RIG-3
Department/Centre: Division of Biological Sciences > Centre for Neuroscience
Date Deposited: 28 Jan 2020 10:46
Last Modified: 28 Jan 2020 10:46
URI: http://eprints.iisc.ac.in/id/eprint/64415

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