Richardson, Peter M and Iali, Wissam and Roy, Soumya S and Rayner, Peter J and Halse, Meghan E and Duckett, Simon B (2019) Rapid C-13 NMR hyperpolarization delivered from para-hydrogen enables the low concentration detection and quantification of sugars. In: CHEMICAL SCIENCE, 10 (45). pp. 10607-10619.
|
PDF
CHE_SCI_10-45_10607_2019.pdf - Published Version Download (1MB) | Preview |
|
|
PDF
c9sc03450a1.pdf - Published Supplemental Material Download (2MB) | Preview |
Abstract
Monosaccharides, such as glucose and fructose, are important to life. In this work we highlight how the rapid delivery of improved C-13 detectability for sugars by nuclear magnetic resonance (NMR) can be achieved using the para-hydrogen based NMR hyperpolarization method SABRE-Relay (Signal Amplification by Reversible Exchange-Relay). The significant C-13 signal enhancements of 250 at a high field of 9.4 T, and 3100 at a low field of 1 T, enable the detection of trace amounts of these materials as well as the quantification of their tautomeric makeup. Using studies on C-13 and H-2 isotopically labelled agents we demonstrate how hyperpolarization lifetime (T-1) values can be extended, and how singlet states with long lifetimes can be created. The precise quantification of d-glucose-C-13(6)-d(7) at the millimolar concentration level is shown to be possible within minutes in conjunction with a linear hyperpolarized response as a function of concentration. In addition to the measurements using labelled materials, low concentration detection is also illustrated for millimolar samples with natural abundance C-13 where isomeric form quantification can be achieved with a single transient.
| Item Type: | Journal Article |
|---|---|
| Publication: | CHEMICAL SCIENCE |
| Publisher: | ROYAL SOC CHEMISTRY |
| Additional Information: | Copyright of this aricle belongs to Royal Society Chemistry |
| Department/Centre: | Division of Chemical Sciences > Inorganic & Physical Chemistry |
| Date Deposited: | 24 Dec 2019 10:44 |
| Last Modified: | 24 Dec 2019 10:44 |
| URI: | http://eprints.iisc.ac.in/id/eprint/64085 |
Actions (login required)
 |
View Item |
