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Remarkably selective biocompatible turn-on fluorescent probe for detection of Fe3+ in human blood samples and cells

Vishaka, V H and Saxena, Manav and Balakrishna, Geetha R and Latiyan, Sachin and Jain, Shilpee (2019) Remarkably selective biocompatible turn-on fluorescent probe for detection of Fe3+ in human blood samples and cells. In: RSC ADVANCES, 9 (47). pp. 27439-27448.

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Official URL: https://dx.doi.org/10.1039/c9ra05256a

Abstract

The robust nature of a biocompatible fluorescent probe is demonstrated, by its detection of Fe3+ even after repeated rounds of quenching (reversibility) by acetate in real human blood samples and cells in vitro. Significantly trace levels of Fe3+ ions up to 8.2 nM could be detected, remaining unaffected by the existence of various other metal ions. The obtained results are validated by AAS and ICP-OES methods. A portable test strip is also fabricated for quick on field detection of Fe3+. As iron is a ubiquitous metal in cells and plays a prominent role in biological processes, the use of this probe to image Fe3+ in cells is a substantial development towards biosensing. Cytotoxicity studies also proved the nontoxic nature of this probe.

Item Type: Journal Article
Publication: RSC ADVANCES
Publisher: ROYAL SOC CHEMISTRY
Additional Information: copyright for this article belongs to ROYAL SOC CHEMISTRY
Department/Centre: Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering
Date Deposited: 22 Oct 2019 11:27
Last Modified: 22 Oct 2019 11:27
URI: http://eprints.iisc.ac.in/id/eprint/63784

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