Vishaka, V H and Saxena, Manav and Balakrishna, Geetha R and Latiyan, Sachin and Jain, Shilpee (2019) Remarkably selective biocompatible turn-on fluorescent probe for detection of Fe3+ in human blood samples and cells. In: RSC ADVANCES, 9 (47). pp. 27439-27448.
|
PDF
rsc_adv_9-47_27439_2019.pdf - Published Version Download (1MB) | Preview |
|
|
PDF
c9ra05256a1.pdf - Published Supplemental Material Download (1MB) | Preview |
Abstract
The robust nature of a biocompatible fluorescent probe is demonstrated, by its detection of Fe3+ even after repeated rounds of quenching (reversibility) by acetate in real human blood samples and cells in vitro. Significantly trace levels of Fe3+ ions up to 8.2 nM could be detected, remaining unaffected by the existence of various other metal ions. The obtained results are validated by AAS and ICP-OES methods. A portable test strip is also fabricated for quick on field detection of Fe3+. As iron is a ubiquitous metal in cells and plays a prominent role in biological processes, the use of this probe to image Fe3+ in cells is a substantial development towards biosensing. Cytotoxicity studies also proved the nontoxic nature of this probe.
| Item Type: | Journal Article |
|---|---|
| Publication: | RSC ADVANCES |
| Publisher: | ROYAL SOC CHEMISTRY |
| Additional Information: | copyright for this article belongs to ROYAL SOC CHEMISTRY |
| Department/Centre: | Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering |
| Date Deposited: | 22 Oct 2019 11:27 |
| Last Modified: | 22 Oct 2019 11:27 |
| URI: | http://eprints.iisc.ac.in/id/eprint/63784 |
Actions (login required)
 |
View Item |
