Rhizospheric life of Salmonella requires flagella-driven motility and EPS-mediated attachment to organic matter and enables cross-kingdom invasion

Karmakar, Kapudeep and Nair, Abhilash Vijay and Chandrasekharan, Giridhar and Garai, Preeti and Nath, Utpal and Nataraj, Karaba N and Prakash, N B and Chakrayortty, Dipshikha (2019) Rhizospheric life of Salmonella requires flagella-driven motility and EPS-mediated attachment to organic matter and enables cross-kingdom invasion. In: FEMS MICROBIOLOGY ECOLOGY, 95 (8).

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Abstract

Salmonella is an established pathogen of the members of the kingdom Animalia. Reports indicate that the association of Salmonella with fresh, edible plant products occurs at the pre-harvest state, i.e. in the field. In this study, we follow the interaction of Salmonella Typhimurium with the model plant Arabidopsis thaliana to understand the process of migration in soil. Plant factors like root exudates serve as chemo-attractants. Our ex situ experiments allowed us to track Salmonella from its free-living state to the endophytic state. We found that genes encoding two-component systems and proteins producing extracellular polymeric substances are essential for Salmonella to adhere to the soil and roots. To understand the trans-kingdom flow of Salmonella, we fed the contaminated plants to mice and observed that it invades and colonizes liver and spleen. To complete the disease cycle, we re-established the infection in plant by mixing the potting mixture with the fecal matter collected from the diseased animals. Our experiments revealed a cross-kingdom invasion by the pathogen via passage through a murine intermediate, a mechanism for its persistence in the soil and invasion in a non-canonical host. These results form a basis to break the life-cycle of Salmonella before it reaches its animal host and thus reduce Salmonella contamination of food products.

Item Type:	Journal Article
Publication:	FEMS MICROBIOLOGY ECOLOGY
Publisher:	OXFORD UNIV PRESS
Additional Information:	copyright for this article belongs to OXFORD UNIV PRESS
Keywords:	Salmonella Typhimurium; flagella; organic matter; EPS; soil; cross-kingdom
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	17 Oct 2019 11:02
Last Modified:	17 Oct 2019 11:02
URI:	http://eprints.iisc.ac.in/id/eprint/63699

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