Recombinant idiotypic $TCR\beta$ chain immunization in mice generates antigen specific T cell response

Lal, Girdhari and Shaila, MS and Nayak, Rabindranath (2006) Recombinant idiotypic $TCR\beta$ chain immunization in mice generates antigen specific T cell response. In: Molecular Immunology, 43 (10). pp. 1549-1556.

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Abstract

Vaccination remains the most cost-effective means of preventing infectious diseases. Success of vaccination depends on generation of effective memory response. Understanding the mechanism of generation and maintenance of immunological memory would help in the design of rational vaccines. T lymphocytes play a central role in the generation of protective immune response against many microbial infections. A hypothesis known as relay hypothesis was earlier proposed, which explains the maintenance of immunological memory through interaction of idiotypic and anti-idiotypic lymphocytes. In the present study, we have shown that immunization with a model antigen, chicken ovalbumin specific T cell receptor beta chain (idiotypic TCR) generates TCR specific antibody and anti-idiotypic T cell responses as well as ovalbumin specific T cell response. We further show that boosting of ovalbumin primed mice with ovalbumin specific idiotypic $TCR\beta$ DNA or $TCR\beta$ protein gives memory response for ovalbumin. This study provides experimental evidence for perpetuation of immunological memory through idiotypic network interactions.

Item Type:	Journal Article
Publication:	Molecular Immunology
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier.
Keywords:	Anti-idiotypic T cells;DNA vaccine;T cell receptor;T cell memory;Relay hypothesis
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	20 Apr 2006
Last Modified:	19 Sep 2010 04:25
URI:	http://eprints.iisc.ac.in/id/eprint/6354

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