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Microfluidic In-Flow Decantation Technique Using Stepped Pillar Arrays and Hydraulic Resistance Tuners

Eluru, Gangadhar and Nagendra, Pavan and Gorthi, Sai Siva (2019) Microfluidic In-Flow Decantation Technique Using Stepped Pillar Arrays and Hydraulic Resistance Tuners. In: MICROMACHINES, 10 (7).

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Official URL: https://dx.doi.org/10.3390/mi10070471


Separating the particles from the liquid component of sample solutions is important for several microfluidic-based sample preparations and/or sample handling techniques, such as plasma separation from whole blood, sheath-free flow focusing, particle enrichment etc. This paper presents a microfluidic in-flow decantation technique that provides the separation of particles from particle-free fluid while in-flow. The design involves the expansion of sample fluid channel in lateral and depth directions, thereby producing a particle-free layer towards the walls of the channel, followed by gradual extraction of this particle-free fluid through a series of tiny openings located towards one-end of the depth-direction. The latter part of this design is quite crucial in the functionality of this decantation technique and is based on the principle called wee-extraction. The design, theory, and simulations were presented to explain the principle-of-operation. To demonstrate the proof-of-principle, the experimental characterization was performed on beads, platelets, and blood samples at various hematocrits (2.5%-45%). The experiments revealed clog-free separation of particle-free fluid for at least an hour of operation of the device and demonstrated purities close to 100% and yields as high as 14%. The avenues to improve the yield are discussed along with several potential applications.

Item Type: Journal Article
Publisher: MDPI
Additional Information: copyright for this article belongs to MDPI
Keywords: in-flow decantation; self-sheath generation; microfluidics; sheath-free flow focusing; plasma separation; particle enrichment
Department/Centre: Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited: 06 Sep 2019 06:20
Last Modified: 06 Sep 2019 06:20
URI: http://eprints.iisc.ac.in/id/eprint/63499

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