Prasad, Deepika and Arora, Divya and Nandicoori, Vinay Kumar and Muniyappa, K (2019) Elucidating the functional role of Mycobacterium smegmatis recX in stress response. In: SCIENTIFIC REPORTS, 9 .
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Abstract
The RecX protein has attracted considerable interest because the recX mutants exhibit multiple phenotypes associated with RecA functions. To further our understanding of the functional relationship between recA and recX, the effect of different stress treatments on their expression profiles, cell yield and viability were investigated. A significant correlation was found between the expression of Mycobacterium smegmatis recA and recX genes at different stages of growth, and in response to different stress treatments albeit recX exhibiting lower transcript and protein abundance at the mid-log and stationary phases of the bacterial growth cycle. To ascertain their roles in vivo, a targeted deletion of the recX and recArecX was performed in M. smegmatis. The growth kinetics of these mutant strains and their sensitivity patterns to different stress treatments were assessed relative to the wild-type strain. The deletion of recA affected normal cell growth and survival, while recX deletion showed no significant effect. Interestingly, deletion of both recX and recA genes results in a phenotype that is intermediate between the phenotypes of the Delta recA mutant and the wild-type strain. Collectively, these results reveal a previously unrecognized role for M. smegmatis recX and support the notion that it may regulate a subset of the yet unknown genes involved in normal cell growth and DNA-damage repair.
| Item Type: | Journal Article |
|---|---|
| Publication: | SCIENTIFIC REPORTS |
| Publisher: | NATURE PUBLISHING GROUP |
| Additional Information: | copyright for this article belongs to NATURE PUBLISHING GROUP |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 13 Aug 2019 10:02 |
| Last Modified: | 13 Aug 2019 10:02 |
| URI: | http://eprints.iisc.ac.in/id/eprint/63403 |
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