Coupling Electrochemical Adsorption and Long-range Electron Transfer: Label-free DNA Mismatch Detection with Ultramicroelectrode (UME)

Basu, Nivedita and Ho, Thu Huong and Guillon, Francois-Xavier and Zhang, Yuanyuan and Bigey, Pascal and Navakanta, Bhat and Bedioui, Fethi and Lazerges, Mathieu (2019) Coupling Electrochemical Adsorption and Long-range Electron Transfer: Label-free DNA Mismatch Detection with Ultramicroelectrode (UME). In: ELECTROANALYSIS .

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Abstract

A new electrochemical hybridization transduction pathway, obtained by coupling electrochemical adsorption and long-range electron transfer through double-stranded DNA, was investigated using ultramicroelectrode (UME). The results show that long-range electron transfer does not occurs exclusively throws well-packed and organized self-assembled DNA monolayers. This approach is used to investigate long-range electron transfer properties of both single- and double- stranded short synthetic DNA and DNA plasmids. Single mismatch electrochemical detection protocol of non-labelled short synthetic DNA, without heating or probe labelling, in a 10 minutes protocol, was in fine performed.

Item Type:	Journal Article
Publication:	ELECTROANALYSIS
Publisher:	WILEY-V C H VERLAG GMBH
Additional Information:	copyright for this article belongs to WILEY-V C H VERLAG GMBH
Department/Centre:	Division of Electrical Sciences > Electrical Communication Engineering Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited:	05 Aug 2019 08:58
Last Modified:	05 Aug 2019 08:58
URI:	http://eprints.iisc.ac.in/id/eprint/63371

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