A Single Atom Change Facilitates the Membrane Transport of Green Fluorescent Proteins in Mammalian Cells

Jakka, Surendar R and Govindaraj, Vijayakumar and Mugesh, Govindasamy (2019) A Single Atom Change Facilitates the Membrane Transport of Green Fluorescent Proteins in Mammalian Cells. In: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 58 (23). pp. 7713-7717.

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Abstract

Direct delivery of proteins into mammalian cells is a challenging problem in biological and biomedical applications. The most common strategies for the delivery of proteins into the cells include the use of cell-penetrating peptides or supercharged proteins. Herein, we show for the first time that a single atom change, hydrogen to halogen, at one of the tyrosine residues can increase the cellular entry of similar to 28kDa green fluorescent protein (GFP) in mammalian cells. The protein uptake is facilitated by a receptor-mediated endocytosis and the cargo can be released effectively into cytosol by co-treatment with the endosomolytic peptide ppTG21.

Item Type:	Journal Article
Publication:	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Publisher:	WILEY-V C H VERLAG GMBH
Additional Information:	copyright for this article belongs to WILEY-V C H VERLAG GMBH
Keywords:	endocytosis; green fluorescent protein; halogens; membrane transport; tRNA synthetase
Department/Centre:	Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	28 Aug 2019 10:05
Last Modified:	28 Aug 2019 10:05
URI:	http://eprints.iisc.ac.in/id/eprint/63327

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