Probing the Formation of a Seleninic Acid in Living Cells by the Fluorescence Switching of a Glutathione Peroxidase Mimetic

Ungati, Harinarayana and Govindaraj, Vijayakumar and Narayanan, Megha and Mugesh, Govindasamy (2019) Probing the Formation of a Seleninic Acid in Living Cells by the Fluorescence Switching of a Glutathione Peroxidase Mimetic. In: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 58 (24). pp. 8156-8160.

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Abstract

Glutathione peroxidase (GPx) is a selenoenzyme that protects cells against oxidative damage. Although the formation of a seleninic acid (-SeO2H) by this enzyme during oxidative stress has been proposed, a selenic acid has not been identified in cells. Herein, we report that the formation of a seleninic acid can be monitored in living cells by using a redox-active ebselen analogue with a naphthalimide fluorophore. The probe reacts with H2O2 to generate the highly fluorescent seleninic acid. The electron withdrawing nature of the -SeO2H moiety and strong SeO interactions, which prevent the photoinduced electron transfer, are responsible for the fluorescence.

Item Type:	Journal Article
Publication:	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Publisher:	WILEY-V C H VERLAG GMBH
Additional Information:	copyright for this article belongs to WILEY-V C H VERLAG GMBH
Keywords:	antioxidant; ebselen; fluorescent probe; glutathione peroxidase; selenium
Department/Centre:	Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	23 Aug 2019 10:05
Last Modified:	23 Aug 2019 10:05
URI:	http://eprints.iisc.ac.in/id/eprint/63326

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