Sirtuin 6 mediated stem cell cardiomyogenesis on protein coated nanofibrous scaffolds

Ghosh, Lopamudra Das and Ravi, Venkatraman and Jain, Aditi and Panicker, Arpana G and Sundaresan, Nagalingam R and Chatterjee, Kaushik (2019) Sirtuin 6 mediated stem cell cardiomyogenesis on protein coated nanofibrous scaffolds. In: NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 19 . pp. 145-155.

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Abstract

The cellular niche provides combination of biomolecular and biophysical cues to control stem cell fate. Three-dimensional (3D) aligned nanofibrous scaffolds can effectively augment stem cell cardiomyogenesis. This work aims to understand the role of biomolecular signals from extracellular matrix (ECM) proteins and leverage them to further promote cardiomyogenesis on nanofibrous scaffolds. Human mesenchymal stem cells (hMSCs) were cultured on 3D aligned polycaprolactone scaffolds coated with different ECM proteins. Among multiple coatings tested, collagen coated fibers were most effective in promoting cardiomyogenesis as determined from increased expression of cardiac biomarkers and intracellular calcium flux. At molecular level, enhanced differentiation on collagen coated fibers was associated with an increased level of sirtuin 6 (SIRT6). Depletion of SIRT6 using siRNA attenuated the differentiation process through activation of Wnt signaling pathway. This study, thus, demonstrates that protein coated scaffolds can augment cardiomyogenic differentiation of stem cells through a combination of topographical and biomolecular signals.

Item Type:	Journal Article
Publication:	NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Publisher:	ELSEVIER SCIENCE BV
Additional Information:	copyright for this article belongs to ELSEVIER SCIENCE BV
Keywords:	Extracellular matrix; Stem cells; Cardiomyogenesis; Tissue scaffolds; Sirtuin6; Nanofibers
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	07 Nov 2019 10:33
Last Modified:	07 Nov 2019 10:33
URI:	http://eprints.iisc.ac.in/id/eprint/63221

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