Circulating Tumor Cell cluster phenotype allows monitoring response to treatment and predicts survival

Balakrishnan, Ajay and Koppaka, Deepak and Anand, Abhishek and Deb, Barnali and Grenci, Gianluca and Viasnoff, Virgile and Thompson, Erik W. and Gowda, Harsha and Bhat, Ramray and Rangarajan, Annapoorni and Thiery, Jean Paul and Babu, K. Govind and Kumar, Prashant (2019) Circulating Tumor Cell cluster phenotype allows monitoring response to treatment and predicts survival. In: SCIENTIFIC REPORTS, 9 .

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Abstract

Circulating tumor cells (CTCs) are putative markers of tumor prognosis and may serve to evaluate patient's response to chemotherapy. CTCs are often detected as single cells but infrequently as clusters and are indicative of worse prognosis. In this study, we developed a short-term culture of nucleated blood cells which was applied to blood samples from breast, lung, esophageal and bladder cancer patients. Clusters of different degrees of compactness, classified as very tight, tight and loose were observed across various cancer types. These clusters show variable expression of cytokeratins. Cluster formation from blood samples obtained during the course of chemotherapy was found to be associated with disease progression and shorter overall survival. The short-term cultures offer a robust and highly reliable method for early prediction of treatment response in different cancer types.

Item Type:	Journal Article
Publication:	SCIENTIFIC REPORTS
Publisher:	NATURE PUBLISHING GROUP
Additional Information:	Copyright of this article belongs to NATURE PUBLISHING GROUP
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	25 Jun 2019 18:59
Last Modified:	25 Jun 2019 18:59
URI:	http://eprints.iisc.ac.in/id/eprint/63055

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