Physical and functional interaction between nucleoid-associated proteins HU and Lsr2 of Mycobacterium tuberculosis: altered DNA binding and gene regulation

Datta, Chandreyee and Jha, Rajiv Kumar and Ahmed, Wareed and Ganguly, Sohini and Ghosh, Soumitra and Nagaraja, Valakunja (2019) Physical and functional interaction between nucleoid-associated proteins HU and Lsr2 of Mycobacterium tuberculosis: altered DNA binding and gene regulation. In: MOLECULAR MICROBIOLOGY, 111 (4). pp. 981-994.

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Abstract

Nucleoid-associated proteins (NAPs) in bacteria contribute to key activities such as DNA compaction, chromosome organization and regulation of gene expression. HU and Lsr2 are two principal NAPs in Mycobacterium tuberculosis (Mtb). HU is essential for Mtb survival and is one of the most abundant NAPs. It differs from other eubacterial HU proteins in having a long, flexible lysine- and arginine-rich carboxy-terminal domain. Lsr2 of Mtb is the functional analogue of the bacterial NAP commonly called H-NS. Lsr2 binds to and regulates expression of A/T-rich portions of the otherwise G/C-rich mycobacterial chromosome. Here, we demonstrate that HU and Lsr2 interact to form a complex. The interaction occurs primarily through the flexible carboxy-terminal domain of HU and the acidic amino-terminal domain of Lsr2. The resulting complex, upon binding to DNA, forms thick nucleoprotein rods, in contrast to the DNA bridging seen with Lsr2 and the DNA compaction seen with HU. Furthermore, transcription assays indicate that the HU-Lsr2 complex is a regulator of gene expression. This physical and functional interaction between two NAPs, which has not been reported previously, is likely to be important for DNA organization and gene expression in Mtb and perhaps other bacterial species.

Item Type:	Journal Article
Publication:	MOLECULAR MICROBIOLOGY
Publisher:	WILEY
Additional Information:	The copyright for this article belongs to Blackwell Publishing Ltd
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	11 May 2019 06:34
Last Modified:	11 May 2019 06:34
URI:	http://eprints.iisc.ac.in/id/eprint/62608

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