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Live cell super resolution imaging by radial fluctuations using fluorogen binding tags

Venkatachalapathy, Muthukumaran and Belapurkar, Vivek and Jose, Mini and Gautier, Arnaud and Nair, Deepak (2019) Live cell super resolution imaging by radial fluctuations using fluorogen binding tags. In: NANOSCALE, 11 (8). pp. 3626-3632.

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Official URL: https://doi.org/10.1039/c8nr07809b


Fluorescence-Activating and absorption-Shifting Tag (FAST) is a novel genetically encoded optical highlighter probe. Since the fluorescence of FAST originates from the stochastic and reversible diffusive association of a fluorogenic ligand, we investigate the application of FAST using Super-Resolution Radial Fluctuations (SRRF) to achieve routine imaging below the diffraction limit in a widefield epifluorescence microscope. We show that intensity fluctuation analysis like SRRF allows the imaging of FAST-tagged proteins with sub - 100 nm resolution in live cells. FAST co-labeled with conventional fluorophores enables real time multicolour 2D and 3D super-resolution imaging, indicating that FAST can be used for the observation of sub-diffraction limited structures in both living and fixed samples.

Item Type: Journal Article
Publication: NANOSCALE
Additional Information: Copyright for this article belongs to RSC.
Department/Centre: Division of Biological Sciences > Centre for Neuroscience
Date Deposited: 19 Mar 2019 08:56
Last Modified: 19 Mar 2019 08:56
URI: http://eprints.iisc.ac.in/id/eprint/61930

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