Electrochemical paper based cancer biosensor using iron oxide nanoparticles decorated PEDOT:PSS

Kumar, Saurabh and Umar, Mohammad and Saifi, Anas and Kumar, Suveen and Augustine, Shine and Srivastava, Saurabh and Malhotra, Bansi D (2019) Electrochemical paper based cancer biosensor using iron oxide nanoparticles decorated PEDOT:PSS. In: ANALYTICA CHIMICA ACTA, 1056 . pp. 135-145.

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Abstract

We report results of the studies relating to the fabrication of a label-free, flexible, light weight and disposable conducting paper based immunosensing platform comprising of poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) and nanostructured iron oxide (nFe(2)O(3)@PEDOT:PSS) nanocomposite for detection of carcinoembryonic antigen (CEA), a cancer biomarker. The effect of various solvents such as sorbitol, ethanol, propanol, n-methyl-2-pyrrolidone (NMP) and dimethyl sulfoxide (DMSO) on the electrical conductivity of Whatman filter paper (WP) modified with nFe(2)O(3)@PEDOT:PSS/WP was investigated. The electrical conductivity of the PEDOT:PSS/WP electrode was found to be enhanced by two orders of magnitude (from 6.8 x 10(-4) to 1.92 x 10(-2) Scm(-1)) after its treatment with DMSO. Further, nFe(2)O(3) doped PEDOT:PSS/WP electrode exhibited the electrical conductivity as 2.4 x 10(-2) Scm(-1). Besides this, the incorporation of iron oxide nanoparticles (nFe(2)O(3)) into PEDOT:PSS/ WP resulted in improved electrochemical performance and signal stability. This nFe(2)O(3)@ PEDOT:PSS/WP based platform was used for immobilization of the anti-carcinoembronic antigen (anti-CEA) protein for quantitative estimation of cancer biomarker (CEA). The results of electrochemical response studies revealed that this conducting paper based immunoelectrode had a sensitivity of 10.2 mu Ang(-1) mLcm(-2) in the physiological range (4-25 ngmL(-1)) and shelf life of 34 days. Further, the proposed immunoelectrode was validated with conventional ELISA for the detection of CEA in serum samples of cancer patients. (C) 2019 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	ANALYTICA CHIMICA ACTA
Publisher:	ELSEVIER SCIENCE BV
Additional Information:	Copyright for this article belongs to Elsevier.
Keywords:	PEDOT:PSS; Iron oxide nanoparticles; Electrochemical paper; Biosensor
Department/Centre:	Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited:	11 Mar 2019 10:08
Last Modified:	11 Mar 2019 10:08
URI:	http://eprints.iisc.ac.in/id/eprint/61916

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