Prasad, Yedu and Kumar, Ritesh and Chaudhary, Awanish Kumar and Dhanaraju, Rajkumar and Majumdar, Soneya and Rao, Desirazu N (2019) Kinetic and catalytic properties of M.HpyAXVII, a phase-variable DNA methyltransferase from Helicobacter pylori. In: JOURNAL OF BIOLOGICAL CHEMISTRY, 294 (3). pp. 1019-1034.
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The bacterium Helicobacter pylori is one of the most common infectious agents found in the human stomach. H. pylori has an unusually large number of DNA methyltransferases (MTases), prompting speculation that they may be involved in the cancerization of epithelial cells. The mod-4a/4b locus, consisting of the hp1369 and hp1370 ORFs, encodes for a truncated and inactive MTase in H. pylori strain 26695. However, slipped-strand synthesis within the phase-variable polyguanine tract in hp1369 results in expression of an active HP1369-1370 fusion N-6-adenine methyltransferase, designated M.HpyAXVII. Sequence analysis of the mod-4a/4b locus across 74 H. pylori strain genomes has provided insights into the regulation of M.HpyAXVII expression. To better understand the role of M.HpyAXVII in the H. pylori biology, here we cloned and overexpressed the hp1369-70 fusion construct in Escherichia coli BL21(DE3) cells. Results from size-exclusion chromatography and multi-angle light scattering (MALS) analyses suggested that M.HpyAXVII exists as a dimer in solution. Kinetic studies, including product and substrate inhibition analyses, initial velocity dependence between substrates, and isotope partitioning, suggested that M.HpyAXVII catalyzes DNA methylation in an ordered Bi Bi mechanism in which the AdoMet binding precedes DNA binding and AdoMet's methyl group is then transferred to an adenine within the DNA recognition sequence. Altering the highly conserved catalytic motif (DPP(Y/F)) as well as the AdoMet-binding motif (FXGXG) by site-directed mutagenesis abolished the catalytic activity of M.HpyAXVII. These results provide insights into the enzyme kinetic mechanism of M.HpyAXVII. We propose that AdoMet binding conformationally ``primes'' the enzyme for DNA binding.
| Item Type: | Journal Article |
|---|---|
| Publication: | JOURNAL OF BIOLOGICAL CHEMISTRY |
| Publisher: | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| Keywords: | DNA methyltransferase; enzyme kinetics; enzyme mechanism; S-adenosylmethionine (AdoMet); bioinformatics; allosteric regulation; DNA binding; M.HpyAXVII; ordered Bi Bi mechanism; phase variation |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 11 Mar 2019 09:17 |
| Last Modified: | 11 Mar 2019 09:17 |
| URI: | http://eprints.iisc.ac.in/id/eprint/61901 |
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