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Microfluidic-based graphene field effect transistor for femtomolar detection of chlorpyrifos

Islam, Saurav and Shukla, Shruti and Bajpai, Vivek K and Han, Young-Kyu and Huh, Yun Suk and Ghosh, Arindam and Gandhi, Sonu (2019) Microfluidic-based graphene field effect transistor for femtomolar detection of chlorpyrifos. In: SCIENTIFIC REPORTS, 9 .

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Official URL: https://doi.org/10.1038/s41598-018-36746-w


Chlorpyrifos is one of the most widely used pesticides that acts on the nervous system by inhibiting acetylcholinesterase. Prolonged use of chlorpyrifos causes severe neurological, autoimmune, and persistent developmental disorders in humans. Therefore, in this study, a highly sensitive and robust biosensor platform was devised by fabricating graphene field effect transistors (graFET) on Si/SiO2 substrate for the detection of chlorpyrifos in real samples. Anti-chlorpyrifos antibodies were immobilized successfully on the graphene surface. Under optimal conditions, graFET sensor showed an excellent response for chlorpyrifos detection in the linear range of 1 fM to 1 mu M with a limit of detection up to 1.8 fM in spiked samples. The developed graFET biosensor is highly stable, sensitive, and specific for chlorpyrifos as confirmed by its significant ability to detect changes in electrostatic potential. These findings signify useful efficacy of immunobiosensors for the detection of chlorpyrifos and other organophosphates in fruits and vegetables.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to NATURE PUBLISHING GROUP
Department/Centre: Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Division of Physical & Mathematical Sciences > Physics
Date Deposited: 14 Feb 2019 05:54
Last Modified: 14 Feb 2019 05:54
URI: http://eprints.iisc.ac.in/id/eprint/61704

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