Gradient platform for combinatorial screening of thermoset polymers for biomedical applications

Dasgupta, Queeny and Madras, Giridhar and Chatterjee, Kaushik (2019) Gradient platform for combinatorial screening of thermoset polymers for biomedical applications. In: MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 94 . pp. 766-777.

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Abstract

The goal of this work was to design a device for rapid screening of crosslinked thermoset polymers. This gradient curing platform is capable of yielding a library of polyesters with systematically varying mechanical and physicochemical properties and the resultant cellular response. A library of poly(xylitolsebacate) polyesters was prepared in this device by differential curing to yield a gradient polymer. The resultant polymer exhibits a gradient in the storage modulus (1 to 5 MPa), wettability (70 degrees < water contact angle < 110 degrees), degree of crosslinking, degradation rate (3-25% in 7 days), drug release and biological response (ability to support stem cell proliferation and differentiation) from one end of the polymer to the other. Primary human mesenchymal stem cells were cultured to assess the cellular response in vitro. Maximal stem cell proliferation and osteogenesis was observed on the highly crosslinked polyester segments that provide high stiffness, are hydrophobic and are slow degrading as compared to the lower cured counterparts. Under in vivo conditions, this material showed differential response across the gradient without displaying significant concerns for inflammation or infection. This gradient curing device is capable of ascertaining suitable curing conditions to obtain appropriate polymers for application specific requirements. This gradient platform was further used to identify optimal processing parameters to prepare three-dimensional tissue scaffolds such as electrospun fiber mats and porous foams. Thus, this versatile combinatorial platform is well suited for rapid screening of thermoset polymers for biomedical applications.

Item Type:	Journal Article
Publication:	MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Publisher:	ELSEVIER SCIENCE BV
Additional Information:	Copyright of this article belong to ELSEVIER SCIENCE BV
Keywords:	Thermal curing; Gradient materials; High throughput screening; Crosslinked polyesters; Biodegradable polymers
Department/Centre:	Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering Division of Mechanical Sciences > Chemical Engineering Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	23 Dec 2018 07:10
Last Modified:	23 Dec 2018 07:10
URI:	http://eprints.iisc.ac.in/id/eprint/61279

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