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Spent embryo culture medium metabolites are related to the in vitro attachment ability of blastocysts

D'Souza, Fiona and Uppangala, Shubhashree and Asampille, Gitanjali and Salian, Sujith Raj and Kalthur, Guruprasad and Talevi, Riccardo and Atreya, Hanudatta S and Adiga, Satish Kumar (2018) Spent embryo culture medium metabolites are related to the in vitro attachment ability of blastocysts. In: SCIENTIFIC REPORTS, 8 .

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Official URL: http://dx.doi.org/10.1038/s41598-018-35342-2


The metabolomic profile of an embryo culture medium can aid in the advanced prediction of embryonic developmental potential and genetic integrity. But it is not known if this technology can be used to determine the in vitro potential of inner cell mass (lCM) in adherence and proliferation. Here, we investigated the developmental potential of mouse 2-cell embryos carrying cisplatin-induced DNA lesions (lDL), beyond blastocyst stage using ICM outgrowth assay. The genetic integrity of ICM cells was determined by comet assay. The metabolic signatures of spent medium were recorded 84 hours post injection of hCG (hpi- hCG), and after 96 hours of extended in vitro culture (Ex 96) by NMR spectroscopy. We observed that blastocysts that lack the ability to adhere in vitro had an increased requirement of pyruvate (p < 0.01), lactate (p < 0.01), and were accompanied by a significant reduction of pyruvatealanine ratio in the culture medium. We propose that the aforementioned metabolites from 84 hpi-hCG spent medium be further explored using appropriate experimental models, to prove their potential as biomarkers in the prediction of implantation ability of in vitro derived human embryos in clinical settings.

Item Type: Journal Article
Department/Centre: Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Division of Chemical Sciences > Solid State & Structural Chemistry Unit
Date Deposited: 19 Dec 2018 15:12
Last Modified: 21 Feb 2019 10:47
URI: http://eprints.iisc.ac.in/id/eprint/61230

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