RecG(wed): A probable novel regulator in the resolution of branched DNA structures in mycobacteria

Singh, Amandeep and Vijayan, M and Nagaraju, Ganesh (2018) RecG(wed): A probable novel regulator in the resolution of branched DNA structures in mycobacteria. In: IUBMB LIFE, 70 (8). pp. 786-794.

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Abstract

Structure-specific helicases, such as RecG, play an important role in the resolution of recombination intermediates. A bioinformatic analysis of mycobacterial genomes led to the identification of a protein (RecG(wed)) with a C-terminal ``edge'' domain, similar to the wedge domain of RecG. RecG(wed) is predominately found in the phylum Actinobacteria and in few human pathogens. Mycobacterium smegmatis RecG(wed) was able to bind branched DNA structures in vitro but failed to interact with single- or double-stranded DNA. The expression of recG(wed) in M. smegmatis cells was up-regulated during stationary phase/UV damage and down-regulated during MMS/H2O2 treatment. These observations indicate the possible involvement of RecG(wed) in transactions during recombination events, that proceed though branched DNA intermediates. (C) 2018

Item Type:	Journal Article
Publication:	IUBMB LIFE
Publisher:	WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Additional Information:	Copy right for this article belong to WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Department/Centre:	Division of Biological Sciences > Biochemistry Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	06 Sep 2018 15:45
Last Modified:	06 Sep 2018 15:45
URI:	http://eprints.iisc.ac.in/id/eprint/60597

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