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TGF-beta induces changes in breast cancer cell deformability

Kulkarni, Ankur H and Chatterjee, Aritra and Kondaiah, Aritra and Kondaiah, Namrata (2018) TGF-beta induces changes in breast cancer cell deformability. In: PHYSICAL BIOLOGY, 15 (6).

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Official URL: http://dx.doi.org/10.1088/1478-3975/aac3ba


Mechanical properties of cells are shown to regulate cell behaviors leading to phenotypic changes that may aid in the development and progression of disease. In this study, we used atomic force microscopy (AFM) indentation with a spherical probe to characterize the elastic and viscoelastic properties of invasive (MDA-MB-231) and noninvasive (MCF-7) breast cancer cells treated with transforming growth factor-beta (TGF-beta). We also used confocal fluorescence imaging to investigate the sub-membrane cytoskeletal structure of the cells. Results showed significant alterations in moduli of both cell types after 24 h TGF-beta treatment which had a context dependent response; moduli for MDA-MB-231 decreased whereas MCF-7 demonstrated stiffening response. Stress relaxation tests showed increased fluid-like nature of MDA-MB-231 following TGF-beta treatment and lower fluidity for MCF-7 cells. We also observed significant alterations in the expression and orientation of actin stress fibers with TGF-beta treatment which correlated with the changes in cell mechanics. Less invasive MCF-7 cells had a delayed overall increase in cell deformability after 48 h exposure to TGF-beta; a similar trend was observed for MDA-MB cells. These changes may be important to facilitate migration, for instance, during metastasis of cancer cells through submicron sized spaces.

Item Type: Journal Article
Additional Information: Copy right for this article belong to IOP PUBLISHING LTD, TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited: 04 Sep 2018 15:41
Last Modified: 28 Nov 2018 15:14
URI: http://eprints.iisc.ac.in/id/eprint/60569

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