Phosphatidylserine and GTPase activation control Cdc42 nanoclustering to counter dissipative diffusion

Sartorel, Elodie and Unlu, Caner and Jose, Mini and Massoni-Laporte, Aurelie and Meca, Julien and Sibarita, Jean-Baptiste and McCusker, Derek (2018) Phosphatidylserine and GTPase activation control Cdc42 nanoclustering to counter dissipative diffusion. In: MOLECULAR BIOLOGY OF THE CELL, 29 (11). pp. 1299-1310.

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Abstract

The anisotropic organization of plasma membrane constituents is indicative of mechanisms that drive the membrane away from equilibrium. However, defining these mechanisms is challenging due to the short spatiotemporal scales at which diffusion operates. Here, we use high-density single protein tracking combined with photoactivation localization microscopy (sptPALM) to monitor Cdc42 in budding yeast, a system in which Cdc42 exhibits anisotropic organization. Cdc42 exhibited reduced mobility at the cell pole, where it was organized in nanoclusters. The Cdc42 nanoclusters were larger at the cell pole than those observed elsewhere in the cell. These features were exacerbated in cells expressing Cdc42-GTP, and were dependent on the scaffold Bem1, which contributed to the range of mobility and nanocluster size exhibited by Cdc42. The lipid environment, in particular phosphatidylserine levels, also played a role in regulating Cdc42 nanoclustering. These studies reveal how the mobility of a Rho GTPase is controlled to counter the depletive effects of diffusion, thus stabilizing Cdc42 on the plasma membrane and sustaining cell polarity.

Item Type:	Journal Article
Publication:	MOLECULAR BIOLOGY OF THE CELL
Publisher:	AMER SOC CELL BIOLOGY, 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA
Additional Information:	Copy right for this article belong to AMER SOC CELL BIOLOGY, 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA
Department/Centre:	Division of Biological Sciences > Centre for Neuroscience
Date Deposited:	27 Aug 2018 13:16
Last Modified:	27 Aug 2018 13:16
URI:	http://eprints.iisc.ac.in/id/eprint/60494

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