Single-molecule fluorescence imaging: Generating insights into molecular interactions in virology

Banerjee, Sunaina and Maurya, Satyaghosh and Roy, Rahul (2018) Single-molecule fluorescence imaging: Generating insights into molecular interactions in virology. In: JOURNAL OF BIOSCIENCES, 43 (3). pp. 519-540.

PDF Jou_Bio_43-3_519_2018.pdf - Published Version Restricted to Registered users only Download (3MB) | Request a copy

Abstract

Single-molecule fluorescence methods remain a challenging yet information-rich set of techniques that allow one to probe the dynamics, stoichiometry and conformation of biomolecules one molecule at a time. Viruses are small (nanometers) in size, can achieve cellular infections with a small number of virions and their lifecycle is inherently heterogeneous with a large number of structural and functional intermediates. Single-molecule measurements that reveal the complete distribution of properties rather than the average can hence reveal new insights into virus infections and biology that are inaccessible otherwise. This article highlights some of the methods and recent applications of single-molecule fluorescence in the field of virology. Here, we have focused on new findings in virus-cell interaction, virus cell entry and transport, viral membrane fusion, genome release, replication, translation, assembly, genome packaging, egress and interaction with host immune proteins that underline the advantage of single-molecule approach to the question at hand. Finally, we discuss the challenges, outlook and potential areas for improvement and future use of single-molecule fluorescence that could further aid our understanding of viruses.

Item Type:	Journal Article
Publication:	JOURNAL OF BIOSCIENCES
Publisher:	INDIAN ACAD SCIENCES, C V RAMAN AVENUE, SADASHIVANAGAR, P B #8005, BANGALORE 560 080, INDIA
Additional Information:	Copyright of this article belong to INDIAN ACAD SCIENCES, C V RAMAN AVENUE, SADASHIVANAGAR, P B #8005, BANGALORE 560 080, INDIA
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering Division of Mechanical Sciences > Chemical Engineering
Date Deposited:	01 Aug 2018 15:22
Last Modified:	25 Aug 2022 11:33
URI:	https://eprints.iisc.ac.in/id/eprint/60342

Actions (login required)

View Item