Applications of STED fluorescence nanoscopy in unravelling nanoscale structure and dynamics of biological systems

Roobala, C and Ilanila, IP and Basu, JK (2018) Applications of STED fluorescence nanoscopy in unravelling nanoscale structure and dynamics of biological systems. In: JOURNAL OF BIOSCIENCES, 43 (3). pp. 471-484.

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Abstract

Fluorescence microscopy, especially confocal microscopy, has revolutionized the field of biological imaging. Breaking the optical diffraction barrier of conventional light microscopy, through the advent of super-resolution microscopy, has ushered in the potential for a second revolution through unprecedented insight into nanoscale structure and dynamics in biological systems. Stimulated emission depletion (STED) microscopy is one such super-resolution microscopy technique which provides real-time enhanced-resolution imaging capabilities. In addition, it can be easily integrated with well-established fluorescence-based techniques such as fluorescence correlation spectroscopy (FCS) in order to capture the structure of cellular membranes at the nanoscale with high temporal resolution. In this review, we discuss the theory of STED and different modalities of operation in order to achieve the best resolution. Various applications of this technique in cell imaging, especially that of neuronal cell imaging, are discussed as well as examples of application of STED imaging in unravelling structure formation on biological membranes. Finally, we have discussed examples from some of our recent studies on nanoscale structure and dynamics of lipids in model membranes, due to interaction with proteins, as revealed by combination of STED and FCS techniques.

Item Type:	Journal Article
Publication:	JOURNAL OF BIOSCIENCES
Publisher:	INDIAN ACAD SCIENCES, C V RAMAN AVENUE, SADASHIVANAGAR, P B #8005, BANGALORE 560 080, INDIA
Additional Information:	Copyright of this article belong to INDIAN ACAD SCIENCES, C V RAMAN AVENUE, SADASHIVANAGAR, P B #8005, BANGALORE 560 080, INDIA
Department/Centre:	Division of Physical & Mathematical Sciences > Physics
Date Deposited:	01 Aug 2018 15:22
Last Modified:	25 Feb 2019 10:35
URI:	http://eprints.iisc.ac.in/id/eprint/60341

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