Koiesa, R S and Joshi, K R and Ancuithasuresh, G K and Sen, P (2018) A NOVEL HIGH THROUGHPUT MICROFLUIDIC CELL TRANSIT ANALYZER USING AIR LIQUID INTERFACE AS LOCALIZED PRESSURE SENSOR. In: 31st IEEE International Conference on Micro Electro Mechanical Systems (MEMS), JAN 21-25, 2018, Belfast, NORTH IRELAND, pp. 133-136.
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Abstract
We report a novel microfluidic platform that measures pressure drop during the passage of a single cell through a mechanical constriction for mechano-phenotyping of cell populations. It uses a precisely positioned Air-Liquid Interface as a Sensor (ALIaS) near the outlet of the constriction. When cells pass through the constriction they momentarily plug the inlet resulting in a drop in the hydrodynamic pressure at the outlet of the constriction. This change in pressure leads to motion of the air-liquid interface which can be correlated to entry and transit time of the cell passage. ALIaS facilitates accurate measurements of cell entry and transit time at high throughout (>200 cells/s). The platform can reliably quantify the variation in deformability in cell populations upon treatment with Glutaraldehyde. The system presents a potential for mechanobiological studies and can be made portable through electrical measurements of interface oscillations.
Item Type: | Conference Proceedings |
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Series.: | Proceedings IEEE Micro Electro Mechanical Systems |
Publisher: | IEEE, 345 E 47TH ST, NEW YORK, NY 10017 USA |
Additional Information: | Copyright of this article belong to IEEE, 345 E 47TH ST, NEW YORK, NY 10017 USA |
Department/Centre: | Division of Mechanical Sciences > Mechanical Engineering Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering |
Date Deposited: | 05 Jul 2018 15:24 |
Last Modified: | 05 Jul 2018 15:24 |
URI: | http://eprints.iisc.ac.in/id/eprint/60156 |
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