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DDX5/p68 associated lncRNA LOC284454 is differentially expressed in human cancers and modulates gene expression

Das, Monalisa and Renganathan, Arun and Dighe, Shrinivas Nivrutti and Bhaduri, Utsa and Shettar, Abhijith and Mukherjee, Geetashree and Kondaiah, Paturu and Rao, Manchanahalli R Satyanarayana (2018) DDX5/p68 associated lncRNA LOC284454 is differentially expressed in human cancers and modulates gene expression. In: RNA BIOLOGY, 15 (2). pp. 214-230.

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Official URL: http://dx.doi.org/10.1080/15476286.2017.1397261


Long non-coding RNAs (lncRNAs) are emerging as important players in regulation of gene expression in higher eukaryotes. DDX5/p68 RNA helicase protein which is involved in splicing of precursor mRNAs also interacts with lncRNAs like, SRA and mrhl, to modulate gene expression. We performed RIP-seq analysis in HEK293T cells to identify the complete repertoire of DDX5/p68 interacting transcripts including 73 single exonic (SE) lncRNAs. The LOC284454 lncRNA is the second top hit of the list of SE lncRNAs which we have characterized in detail for its molecular features and cellular functions. The RNA is located in the same primary transcript harboring miR-23a approximate to 27a approximate to 24-2 cluster. LOC284454 is a stable, nuclear restricted and chromatin associated lncRNA. The sequence is conserved only in primates among 26 different species and is expressed in multiple human tissues. Expression of LOC284454 is significantly reduced in breast, prostate, uterus and kidney cancer and also in breast cancer cell lines (MCF7 and T47D). Global gene expression studies upon loss and gain of function of LOC284454 revealed perturbation of genes related to cancer-related pathways. Focal adhesion and cell migration pathway genes are downregulated under overexpression condition, and these genes are significantly upregulated in breast cancer cell lines as well as breast cancer tissue samples suggesting a functional role of LOC284454 lncRNA in breast cancer pathobiology.

Item Type: Journal Article
Publication: RNA BIOLOGY
Additional Information: Copy right for this article belong to the TAYLOR & FRANCIS INC, 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited: 02 Mar 2018 14:54
Last Modified: 03 Oct 2018 14:15
URI: http://eprints.iisc.ac.in/id/eprint/59062

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