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Conformational heterogeneity in tails of DNA-binding proteins is augmented by proline containing repeats

Khare, Harshavardhan and Dey, Debayan and Madhu, Chilakapati and Senapati, Dillip and Raghothama, Srinivasarao and Govindaraju, Thimmaiah and Ramakumar, Suryanarayanarao (2017) Conformational heterogeneity in tails of DNA-binding proteins is augmented by proline containing repeats. In: MOLECULAR BIOSYSTEMS, 13 (12). pp. 2531-2544.

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Official URL: http://dx.doi.org/10.1039/c7mb00412e


A cationic terminal extension or tail is a common feature of many DNA-binding proteins. We show that a particular type of tail rich in proline, alanine and lysine belongs to the class of `flexible disorder' and consists of characteristic pentapeptide repeats. Our designed peptides, (AAKKA)(1-4) and (PAKKA)(1-4), represent the tails of several bacterial DNA-binding proteins. Enhanced conformational sampling of these representative peptides using accelerated molecular dynamic simulations supported by circular dichroism spectroscopy and nuclear magnetic resonance studies demonstrates the role of frequent and interspersed prolines in augmenting conformational heterogeneity of the peptide backbone. Analysis of circular variance of backbone dihedral angles indicates alternating regions of relative rigidity and flexibility along the peptide sequence due to prolines. Preferred placement of lysines in the regions of higher backbone flexibility might improve DNA-binding by conformational selection. Our results could be relevant for rational de novo design of disordered peptides.

Item Type: Journal Article
Additional Information: Copright for this article belong to the ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Department/Centre: Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Division of Physical & Mathematical Sciences > Physics
Date Deposited: 02 Mar 2018 15:05
Last Modified: 02 Mar 2018 15:05
URI: http://eprints.iisc.ac.in/id/eprint/58927

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