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Investigation into a community outbreak of Salmonella Typhi in Bengaluru, India

Purighalla, Swathi and Esakimuthu, Saritha and Reddy, Mallika and Seth, Tara and Patil, Sunita D and Varghese, George K and Dasarathy, Raghunath and Richard, Vijay S and Sambandamurthy, Vasan K (2017) Investigation into a community outbreak of Salmonella Typhi in Bengaluru, India. In: INDIAN JOURNAL OF MEDICAL RESEARCH, 146 (S). pp. 15-22.

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Official URL: http://dx.doi.org/10.4103/ijmr.IJMR_1201_16


Background & ohjectives: Outbreaks of infection due to Salmonella enterica servovar Typhi (S. Typhi) are a great threat to public health. A rapid molecular typing method to characterize strains implicated in an outbreak is critical in implementing appropriate control measures. This study was done to demonstrate the power of a PCR-based method to provide rapid insights into the genetic relatedness amongst the Salmonella isolates implicated in a suspected typhoid fever outbreak. Methods: Forty two Typhi isolates originating from three geographically distinct areas, with one area suspected to have a single -source outbreak were included in the study. The genetic fingerprint of all isolates was generated using enterobacterial repetitive intergenic consensus sequence based-PCR (ERIC-PCR). The antimicrobial susceptibility profiles were also evaluated. Results: ERIC-PCR was found to be rapid and reproducible with a discriminatory index of 0.766. The dendrogram constructed based on ERIC-PCR fingerprinting revealed the existence of 12 distinct genotypes. The location suspected to have an outbreak displayed two genotypes amongst the 24 isolates. The other two locations (18 isolates) displayed genetic heterogeneity. The clonality of the outbreak isolates from the time-matched control isolates was established. The observed antimicrobial susceptibility profiles did not have any discriminatory power to subtype the isolates compared to the genetic fingerprints. Interpretation & conclusions: Our study demonstrated the discriminatory power and value of ERIC-PCR in the typing of S. Typhi isolates and providing valuable epidemiological insights.

Item Type: Journal Article
Publisher: 10.4103/ijmr.IJMR_1201_16
Additional Information: Copy right for this article belongs to the MEDKNOW PUBLICATIONS & MEDIA PVT LTD, B-9, KANARA BUSINESS CENTRE, OFF LINK RD, GHAKTOPAR-E, MUMBAI, 400075, INDIA
Department/Centre: Autonomous Societies / Centres > Society for Innovation and Development
Date Deposited: 13 Jan 2018 06:08
Last Modified: 06 Nov 2018 07:55
URI: http://eprints.iisc.ac.in/id/eprint/58611

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