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Characterization of a second Single Stranded DNA Binding protein from Mycobacterium smegmatis

Singh, A and Varshney, U and Vijayan, M (2017) Characterization of a second Single Stranded DNA Binding protein from Mycobacterium smegmatis. In: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 46 (1). S147.

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Official URL: http://dx.doi.org/10.1016/j.jsb.2016.09.012


All mycobacteria with sequenced genomes, except M. leprae, have a second Single Stranded DNA Binding protein (SSBb) in addition to the canonical one (SSBa). This paralogue from M. smegmatis (MsSSBb) has been cloned, expressed and purified. The protein, which is probably involved in stress response, has been crystallized and X-ray analyzed in the first structure elucidation of a mycobacterial SSBb. In spite of the low sequence identity between SSBas and SSBbs in mycobacteria, the tertiary and quaternary structure of the DNA binding domain of MsSSBb is similar to that observed in mycobacterial SSBas. In particular, the quaternary structure is 'clamped' using a C-terminal stretch of the N-domain, which endows the tetrameric molecule with additional stability and its characteristic shape. Comparison involving available, rather limited, structural data on SSBbs from other sources, appears to suggest that SSBbs could exhibit higher structural variability than SSBas do.

Item Type: Editorials/Short Communications
Publisher: 10.1016/j.jsb.2016.09.012
Additional Information: 19th IUPAB Congress / 11th EBSA Congress, British Biophys Soc, Edinburgh, SCOTLAND, JUL 16-20, 2017 Copy right for this article belongs to the SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 USA
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 13 Jan 2018 05:43
Last Modified: 13 Jan 2018 05:43
URI: http://eprints.iisc.ac.in/id/eprint/58610

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