Stephen-Victor, Emmanuel and Karnam, Anupama and Fontaine, Thierry and Beauvais, Anne and Das, Mrinmoy and Hegde, Pushpa and Prakhar, Praveen and Holla, Sahana and Balaji, Kithiganahalli N and Kaveri, Srini V and Latge, Jean-Paul and Aimanianda, Vishukumar and Bayry, Jagadeesh (2017) Aspergillus fumigatus Cell Wall alpha-(1,3)-Glucan Stimulates Regulatory T-Cell Polarization by Inducing PD-L1 Expression on Human Dendritic Cells. In: JOURNAL OF INFECTIOUS DISEASES, 216 (10). pp. 1281-1294.
Full text not available from this repository. (Request a copy)Abstract
Background. Human dendritic cell (DC) response to alpha-(1,3)-glucan polysaccharide of Aspergillus fumigatus and ensuing CD4(+) T-cell polarization are poorly characterized. Methods. alpha-(1,3)-Glucan was isolated from A. fumigatus conidia and mycelia cell wall. For the analysis of polarization, DCs and autologous naive CD4(+) T cells were cocultured. Phenotype of immune cells was analyzed by flow cytometry, and cytokines by enzyme-linked immunosorbent assay (ELISA). Blocking antibodies were used to dissect the role of Toll-like receptor 2 (TLR2) and programmed death-ligand 1 (PD-L1) in regulating alpha-(1,3)-glucan-mediated DC activation and T-cell responses. DCs from TLR2-deficient mice were additionally used to consolidate the findings. Results. alpha-(1,3)-Glucan induced the maturation of DCs and was dependent in part on TLR2. ``alpha-(1,3)-Glucan-educated'' DCs stimulated the activation of naive T cells and polarized a subset of these cells into CD4(+)CD25(+)FoxP3(+) regulatory T cells (Tregs). Mechanistically, Treg stimulation by alpha-(1,3)-glucan was dependent on the PD-L1 pathway that negatively regulated interferon-gamma (IFN-gamma) secretion. Short alpha-(1,3)-oligosaccharides lacked the capacity to induce maturation of DCs but significantly blocked alpha-(1,3)-glucan-induced Treg polarization. Conclusions. PD-L1 dictates the balance between Treg and IFN-gamma responses induced by alpha-(1,3)-glucan. Our data provide a rationale for the exploitation of immunotherapeutic approaches that target PD-1-PD-L1 to enhance protective immune responses to A. fumigatus infections.
| Item Type: | Journal Article |
|---|---|
| Publication: | JOURNAL OF INFECTIOUS DISEASES |
| Publisher: | 10.1093/infdis/jix469 |
| Additional Information: | Copy right for this article belongs to the OXFORD UNIV PRESS INC, JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA |
| Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
| Date Deposited: | 13 Jan 2018 06:35 |
| Last Modified: | 13 Jan 2018 06:35 |
| URI: | http://eprints.iisc.ac.in/id/eprint/58597 |
Actions (login required)
 |
View Item |
