Development of cotton transgenics with antisense AV2 gene for resistance against cotton leaf curl virus (CLCuD) via Agrobacterium tumefaciens

Sanjaya, * and Satyavathi, VV and Prasad, V and Kirthi, N and Maiya, SP and Savithri, HS and Sita, Lakshmi G (2005) Development of cotton transgenics with antisense AV2 gene for resistance against cotton leaf curl virus (CLCuD) via Agrobacterium tumefaciens. In: Plant Cell, Tissue and Organ Culture, 81 (1). pp. 55-63.

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Abstract

Cotton transgenics for resistance against cotton leaf curl disease using antisense movement protein gene (AV2) were developed in an Indian variety (F846) via Agrobacterium-mediated transformation using the protocol developed previously. A binary vector pPZP carrying the antisense AV2 (350 bp) gene along with the nptII gene was used. Transgenic nature of the putative transgenics was confirmed by molecular analysis. Shoots were induced on selection medium and subcultured on rooting medium contg. IBA and $75 mg \hspace {3mm} l^ {-1}$ kanamycin. Transgenic plants were recovered in 12-16 weeks from the time of gene transfer to establishment in pots. Preliminary analysis of the field-established plantlets was conducted by PCR. $T_1$ plants were obtained from $T_0$ seeds, the presence of the AV2 and nptII genes in the transgenic plants was verified by PCR and integration of T-DNA with AV2 into the plant genome of putative transgenics was further confirmed by Southern blot analysis. Several $T_1$ lines were maintained in the greenhouse. Progeny anal. of these plants by PCR analysis showed a classical Mendelian pattern of inheritance.

Item Type:	Journal Article
Publication:	Plant Cell, Tissue and Organ Culture
Publisher:	Springer
Additional Information:	The Copyright belongs to Springer.
Keywords:	cross protection;Gemini virus;Genetic transformation;Gossypium hirsutum;Virus resistance
Department/Centre:	Division of Biological Sciences > Biochemistry Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	17 Mar 2006
Last Modified:	19 Sep 2010 04:24
URI:	http://eprints.iisc.ac.in/id/eprint/5818

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