Dual-Mode Optical Sensing of Histamine at Nanomolar Concentrations in Complex Biological Fluids and Living Cells

Dey, Nilanjan and Ali, Asfa and Podder, Santosh and Majumdar, Shamik and Nandi, Dipankar and Bhattacharya, Santanu (2017) Dual-Mode Optical Sensing of Histamine at Nanomolar Concentrations in Complex Biological Fluids and Living Cells. In: CHEMISTRY-A EUROPEAN JOURNAL, 23 (49). pp. 11891-11897.

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Abstract

An easily synthesized fluorescein-based luminescent dye has been utilized for the dual-mode detection of histamine at nanomolar concentrations at pH7.0 in water. The specific response to histamine was achieved by imidazole-catalyzed imine formation' reaction. The protocol was subsequently applied for the estimation of histamine in complex biological milieu such as human blood serum and urine samples. Furthermore, the dose-dependent cellular uptake of histamine and de novo synthesis (by thapsigargin treatment) was visualized in RAW 264.7, a mouse macrophage cell line. We have also developed portable paper strips for rapid, on-site detection of histamine without involving costly instruments.

Item Type:	Journal Article
Publication:	CHEMISTRY-A EUROPEAN JOURNAL
Additional Information:	Copy right for this article belongs to the WILEY-V C H VERLAG GMBH, POSTFACH 101161, 69451 WEINHEIM, GERMANY
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	23 Sep 2017 05:10
Last Modified:	23 Sep 2017 05:10
URI:	http://eprints.iisc.ac.in/id/eprint/57858

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