Terpyridyl oxovanadium(IV) complexes for DNA crosslinking and mito-targeted photocytotoxicity

Kumar, Arun and Pant, Ila and Dixit, Akanksha and Banerjee, Samya and Banik, Bhabatosh and Saha, Rupak and Kondaiah, Paturu and Chakravarty, Akhil R (2017) Terpyridyl oxovanadium(IV) complexes for DNA crosslinking and mito-targeted photocytotoxicity. In: JOURNAL OF INORGANIC BIOCHEMISTRY, 174 . pp. 45-54.

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Abstract

Oxovanadium(IV) complexes VO(L-1/L-2)Cl-2](n+) (1,2) of (anthracenyl)terpyridine (An-tpy as L-1 in 1, n = 0) and triphenylphosphonium-appended (anthracenyl)terpyridine (An-tpy-TPP+ as L-2 in 2, n = 1) were synthesized, characterized and their DNA crosslinking ability, photocytotoxicity in visible light and cellular localization in cancer cells studied. The bromide derivative of 2, viz. VO(An-tpy-TPP)Br-2]Br (3) is structurally characterized. The structure showed trans disposition of two halides in the coordination sphere and the TPP+ unit is a pendant to the terpyridyl ligand. The DNA melting and comet assay studies on the complexes suggest the formation of DNA crosslinks. Complexes 1 and 2 displayed similar to 10 fold increase in cytotoxicity on exposure to visible light (400-700 nm) when compared to those in dark in HeLa and MCF-7 cells. FACScan (Fluorescence Associated Cell Sorter Scan) analysis showed cellular apoptosis when treated with the complex in visible light in comparison to their dark controls. Fluorescence microscopic studies using complex 2 revealed its mitochondrial localization within the cancer cells.

Item Type:	Journal Article
Publication:	JOURNAL OF INORGANIC BIOCHEMISTRY
Additional Information:	Copy right for this article belongs to the ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
Department/Centre:	Division of Biological Sciences > Biochemistry Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	24 Aug 2017 05:13
Last Modified:	24 Aug 2017 05:13
URI:	http://eprints.iisc.ac.in/id/eprint/57660

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