Synergy of substrate conductivity and intermittent electrical stimulation towards osteogenic differentiation of human mesenchymal stem cells

Ravikumar, K and Boda, Sunil Kumar and Basu, Bikramjit (2017) Synergy of substrate conductivity and intermittent electrical stimulation towards osteogenic differentiation of human mesenchymal stem cells. In: BIOELECTROCHEMISTRY, 116 . pp. 52-64.

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Abstract

Human Mesenchymal Stem cells (hMSCs) have the unique potential to differentiate into multiple cell types. Depending on the cellular microenvironment (physical and biochemical cues), hMSCs can be directed to differentiate into osteogenic, chondrogenic, myogenic and adipogenic lineages. Among the strategies available to direct stem cell fate processes, electrical stimulation based approach has been extensively investigated in recent studies. In the present study, the conducting Hydroxyapatite-CaTiO3 (HA-CT) composites are used as electroconductive platforms to support the differentiation of hMSCs, in vitro. During culture without osteogenic supplements, intermittent electrical stimulation is provided every 24 h over a period of 4 weeks through parallel plate electrodes separated by a distance of 15 mm and maintained at a static potential of 15 V for 10 min. In addition to cell morphological changes, the differentiation behavior of hMSCs after electrical stimulation is evaluated by mRNA expression analysis through polymerase chain reaction (PCR). Importantly, specific bone markers, in particular ALP, Col IA and Osteocalcin are expressed more significantly due to electrical stimulation, which also enhances the extent of extracellular matrix mineralization. Taken together, this study establishes the effectiveness of electroconductive HA-CT composites together with intermittent electrical stimulation to direct osteogenesis of hMSCs. (C) 2017 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	BIOELECTROCHEMISTRY
Additional Information:	Copy right for this article belongs to the ELSEVIER SCIENCE SA, PO BOX 564, 1001 LAUSANNE, SWITZERLAND
Department/Centre:	Division of Chemical Sciences > Materials Research Centre
Date Deposited:	12 Aug 2017 04:14
Last Modified:	03 Nov 2018 08:53
URI:	http://eprints.iisc.ac.in/id/eprint/57598

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