Comparative proteomic analysis of serum of mice infected with Plasmodium berghei ANKH strain using two dimensional gel electrophoresis and matrix-assisted laser desorption ionization - time-of-flight mass spectrometry

Vathsala, P G and Murthy, Krishna P (2016) Comparative proteomic analysis of serum of mice infected with Plasmodium berghei ANKH strain using two dimensional gel electrophoresis and matrix-assisted laser desorption ionization - time-of-flight mass spectrometry. In: INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 410 . pp. 57-62.

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Abstract

Malaria, worldwide, affects around 500 million people with 2-3 million clinical cases every year. In this study, a proteomic approach was used to analyze the alterations in mice serum proteome induced by P. berghei infection; and identified number of differentially expressed proteins compared to control. Serum proteins were separated by two-dimensional electrophoresis (2DE). These proteins were further identified by matrix-assisted laser desorption or ionization - time-of-flight (MALDI-TOF) spectrometry. Important proteins identified include Serum amyloid A4 protein, Relch-like protein, Glutathione-S-transferase Mul. The identified proteins could have a significant role in developing a biomarker-based diagnostic tool for malaria. (C) 2016 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
Additional Information:	Copy right for this article belongs to the ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
Department/Centre:	UG Programme
Date Deposited:	31 Jan 2017 05:30
Last Modified:	31 Jan 2017 05:30
URI:	http://eprints.iisc.ac.in/id/eprint/56127

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