Potent anticancer activity of photo-activated oxo-bridged diiron(III) complexes

Chanu, Binita S and Banerjee, Samya and Roy, Mithun (2017) Potent anticancer activity of photo-activated oxo-bridged diiron(III) complexes. In: EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 125 . pp. 816-824.

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Abstract

Cancer-specific anticancer drugs are still an elusive goal. Using light as the temporal control to generate cytotoxic species from photo-activated prodrug in the presence or absence of molecular oxygen has shown potential application targeted chemotherapy as in photodynamic therapy (PDT). In the present work we explored the chemistry of several photo-active (mu-oxo)diiron(III) complexes of the following formulation (Fe(mu-O) (L-his)(B)}(2)](ClO4)(2) (1a-1c), Fe-2(mu-O)(H2O)(2)B-4](ClO4)(4) (2b, 2c) and Fe-2(mu-O)(mu-O2CMe)B-4](ClO4)(3) (3b, 3c), L-his = L-histidine, B is 2,2'-bipyridine, 1,10-phenanthroline (phen) and dipyrido3,2-d:2',3'-f]quinoxaline (dpq) complexes for tumor-specific anticancer activity. Facile redox chemistry and photochemical aspects of the complexes prompted us to investigate the cytotoxic as well as the photo-activated cytotoxic properties of the complexes to the cancer cells. In the present investigation we explored the cancer-specific condition of excess concentration of H2O2 for our approach to targeted chemotherapy. Cytotoxic effect of the complexes to the cancer cells was found to be significantly higher than in normal cells indicating tumor-specific anticancer activity of the complexes. Cytotoxic effect was even more pronounced when the cancer cells treated with the complexes were exposed to the visible light (400-700 nm). There was >12 fold increase in cytotoxicity of the photoactivated complexes in cancer cells (MCF-7) in comparison to the normal cells (MCF-10a). We have defined a factor viz, cancer cell specificity factor (f) describing the targeted photochemotherapeutic effect of the complexes at their specific concentration. The factor (f) > 1 indicated the cancer cell specificity of the complexes, while f > 2.5 for the complexes under the visible light exposure suggested photodynamic effect. DCFDA assay indicated the presence of excess of ROS in the treated HeLa cells. ROS concentration was found to increase even more on visible light exposure. Increased ROS in the cancer cells disturb the cellular redox mechanism inducing oxidative stress to lethality. Decarboxylation of photo-activated diiron(III) complexes generate (OH)-O-center dot radical responsible for cell death. Overall, the high efficacy and selectivity of the (mu-oxo)diiron(III) complexes potentially make them suitable for in vivo applications and extensive testing toward transfer into the clinical arena. (C) 2016 Elsevier Masson SAS. All rights reserved.

Item Type:	Journal Article
Publication:	EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
Additional Information:	Copy right for this article belongs to the ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 23 RUE LINOIS, 75724 PARIS, FRANCE
Department/Centre:	Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	31 Jan 2017 05:27
Last Modified:	31 Jan 2017 05:27
URI:	http://eprints.iisc.ac.in/id/eprint/56035

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