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Structural characterization and comparative biomedical properties of phloroglucinol from Indian brown seaweeds

Karthik, Ramachandran and Manigandan, Venkatesan and Sheeba, Ravi and Saravanan, Ramachandran and Rajesh, Pushpabai Rajaian (2016) Structural characterization and comparative biomedical properties of phloroglucinol from Indian brown seaweeds. In: JOURNAL OF APPLIED PHYCOLOGY, 28 (6). pp. 3561-3573.

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Official URL: http://dx.doi.org/10.1007/s10811-016-0851-2


In the present study, phloroglucinol, a phenolic derivative, was extracted from the brown seaweeds Sargassum wightii, Sargassum tenerrimum and Turbinaria conoides. The secondary metabolite purified by reversed phase-high-performance liquid chromatography (RP-HPLC) was characterized using Fourier transform infrared spectroscopy (FT-IR), fluorescence spectroscopy, matrix-assisted laser desorption/ionization-time of flight/mass spectrometry (MALDI-TOF/MS) and H-1 nuclear magnetic resonance (NMR) spectroscopy. Phloroglucinol from S. tenerrimum exhibited higher total antioxidant (453.3 mg AscAE g(-1)), DPPH-scavenging (71.07 %), H2O2-scavenging (89.7 %) and metal chelating (14.28 %) activities. Anticoagulant activity was maximum in phloroglucinol from T. conoides (APTT 38.2 s, PT 22.7 s and FC 1.92 g dL(-1)). Phloroglucinol from S. wightii showed maximum lymphocyte migration inhibition (0.8 mm). Cytotoxicity and lactate dehydrogenase (LDH) leakage against MCF-7 cells was high in phloroglucinol from T. conoides (IC50 10.74 mu g mL(-1) and 56.17 %). These findings suggest that phloroglucinol from the selected brown seaweeds could be a rich functional food with appreciable biomedical properties.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to theSPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 31 Jan 2017 04:34
Last Modified: 31 Jan 2017 04:34
URI: http://eprints.iisc.ac.in/id/eprint/55971

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