Quantum dot-loaded monofunctionalized DNA icosahedra for single-particle tracking of endocytic pathways

Bhatia, Dhiraj and Arumugam, Senthil and Nasilowski, Michel and Joshi, Himanshu and Wunder, Christian and Chambon, Valerie and Prakash, Ved and Grazon, Chloe and Nadal, Brice and Maiti, Prabal K and Johannes, Ludger and Dubertret, Benoit and Krishnan, Yamuna (2016) Quantum dot-loaded monofunctionalized DNA icosahedra for single-particle tracking of endocytic pathways. In: NATURE NANOTECHNOLOGY, 11 (12). pp. 1112-1119.

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Abstract

Functionalization of quantum dots (QDs) with a single biomolecular tag using traditional approaches in bulk solution has met with limited success. DNA polyhedra consist of an internal void bounded by a well-defined three-dimensional structured surface. The void can house cargo and the surface can be functionalized with stoichiometric and spatial precision. Here, we show that monofunctionalized QDs can be realized by encapsulating QDs inside DNA icosahedra and functionalizing the DNA shell with an endocytic ligand. We deployed the DNA-encapsulated QDs for real-time imaging of three different endocytic ligands-folic acid, galectin-3 (Gal3) and the Shiga toxin B-subunit (STxB). Single-particle tracking of Gal3- or STxB-functionalized QD-loaded DNA icosahedra allows us to monitor compartmental dynamics along endocytic pathways. These DNA-encapsulated QDs, which bear a unique stoichiometry of endocytic ligands, represent a new class of molecular probes for quantitative imaging of endocytic receptor dynamics.

Item Type:	Journal Article
Publication:	NATURE NANOTECHNOLOGY
Additional Information:	Copy right for this article belongs to the NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
Department/Centre:	Division of Physical & Mathematical Sciences > Physics
Date Deposited:	20 Jan 2017 04:16
Last Modified:	20 Jan 2017 04:16
URI:	http://eprints.iisc.ac.in/id/eprint/55918

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