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Effect of Linkage on the Location of Reducing and Nonreducing Sugars Bound to Jacalin

Abhinav, KV and Sharma, Kaushal and Surolia, Avadhesha and Vijayan, Mamannamana (2016) Effect of Linkage on the Location of Reducing and Nonreducing Sugars Bound to Jacalin. In: IUBMB LIFE, 68 (12). pp. 971-979.

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Official URL: http://dx.doi.org/10.1002/iub.1572


The crystal structures of jacalin complexed with Gal alpha-(1,4) Gal and Gal alpha-(1,3) Gal beta-(1,4) Gal have been determined with the primary objective of exploring the effect of linkage on the location of reducing and non-reducing sugars in the extended binding site of the lectin, an issue which has not been studied thoroughly. Contrary to the earlier surmise based on simple steric considerations, the two structures demonstrate that alpha-linked sugars can bind to jacalin with nonreducing sugar at the primary binding site. This is made possible substantially on account of the hitherto underestimated plasticity of a non-polar region of the extended binding site. Modeling studies involving conformational search and energy minimization, along with available crystallographic and thermodynamic data, indicate a strong preference for complexation with Gal beta-(1,3) Gal with the reducing Gal at the primary site, followed by that with Gal alpha-(1,3) Gal, with the reducing or non-reducing Gal located at the primary binding site. This observation is in consonance with the facility of jacalin to bind mucin type O-glycans containing T-antigen core. (C) 2016 IUBMB Life, 68(12): 971-979, 2016

Item Type: Journal Article
Publication: IUBMB LIFE
Additional Information: Copy right for this article belongs to the WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 30 Dec 2016 07:06
Last Modified: 30 Dec 2016 07:06
URI: http://eprints.iisc.ac.in/id/eprint/55630

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