ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Supramolecular Approach to Enzyme Sensing on Paper Discs Using Lanthanide Photoluminescence

Gorai, Tumpa and Maitre, Uday (2016) Supramolecular Approach to Enzyme Sensing on Paper Discs Using Lanthanide Photoluminescence. In: ACS SENSORS, 1 (7). pp. 934-940.

[img] PDF
ACS_Sen_1-7_934_2016.pdf - Published Version
Restricted to Registered users only

Download (2MB) | Request a copy
Official URL: http://dx.doi.org/10.1021/acssensors.6b00341


A paper based photoluminescent biosensor has been developed, with green Tb-luminescence as the output, for the rapid detection of beta-glucosidase and lipase, both in purified form and in biological/natural fluids. A Tb Cholate hydrogel doped with specially designed, specific enzyme substrates (''pro''-sensitizers) was integrated on filter paper discs. The addition of small volumes(similar to 5 mu L) of enzyme solutions on these discs led to enhanced green emission (UV lamp, lambda(ex) 365 nm), allowing qualitative detection of the enzymes-when needed, the intensity enhancement can be quantified using image processing software, or for multiple samples using a commercial plate reader. This simple technique allowed the detection of beta-glucosidase in almond extract and lipase in blood serum. Easy identification of the inhibition of beta-glucosidase was also demonstrated. The paper based sensor is inexpensive, user-friendly and needs low volumes of biological sample for analysis. This strategy has the potential to be useful for possible clinical applications.

Item Type: Journal Article
Publication: ACS SENSORS
Additional Information: Copy right for this article belongs to the AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
Department/Centre: Division of Chemical Sciences > Organic Chemistry
Date Deposited: 03 Dec 2016 09:41
Last Modified: 03 Dec 2016 09:41
URI: http://eprints.iisc.ac.in/id/eprint/55361

Actions (login required)

View Item View Item